Peptide Reconstitution Calculator
Calculate the exact concentration, draw volume, and insulin syringe units for any peptide vial. Enter your vial size and BAC water volume below.
Enter your vial amount, how much BAC water you'll add, and the peptide amount in mcg. We'll tell you exactly how much to draw.
Printed on your vial label
Volume of diluent added
Research amount in micrograms
2.50 mg/mL
2500 mcg/mL
0.100 mL
100.0 ”L
10.0 units
On a 100-unit insulin syringe
How to use this calculator
- 1Enter the amount of peptide in your vial in milligrams (mg). This is printed on the vial label.
- 2Enter the volume of bacteriostatic water (BAC water) you will add to the vial in millilitres (mL).
- 3Select your peptide amount in micrograms (mcg).
- 4The calculator instantly shows the concentration of your solution and the exact volume and syringe units to draw.
- 5Use the Reverse tab if you already know the units drawn and want to check the resulting peptide amount.
Research Use Only
This calculator is provided as a convenience tool for in-vitro research purposes only. All compounds sold by Peptides.net are strictly for laboratory research. This tool does not constitute medical advice.
Common reconstitution volumes
Reconstitution FAQs
What is bacteriostatic water and why do I use it?
Bacteriostatic water (BAC water) is sterile water containing 0.9% benzyl alcohol. The benzyl alcohol acts as a preservative that inhibits bacterial growth, allowing your reconstituted peptide solution to remain stable for up to 28 days when refrigerated. It is the standard diluent for research peptide reconstitution.
How do I reconstitute a lyophilised peptide?
Draw the required volume of BAC water into a syringe. Insert the needle through the rubber stopper of the peptide vial and direct the stream gently down the inside wall of the vial. Do not squirt it directly onto the powder. Once added, gently swirl (do not shake) the vial until the powder is fully dissolved. The solution should be clear.
What does 'units on a syringe' mean?
A standard insulin syringe is marked in units, where 100 units = 1 mL. Each unit therefore equals 0.01 mL (10 microlitres). The calculator converts your required volume (in mL) to units, making it easy to read the correct graduation on an insulin syringe without manual conversion.
How should I store a reconstituted peptide?
Reconstituted peptides should be stored in a refrigerator at 2â8°C and used within 28 days. Avoid freezing a reconstituted solution as this can degrade the peptide. If you do not plan to use a vial within 28 days, it is better to reconstitute only what you need and keep the remainder as lyophilised powder.
What concentration should I reconstitute to?
The ideal concentration depends on the peptide and your research protocol. A common approach is to reconstitute so that each research amount corresponds to a convenient volume. For example, 0.1 mL (10 units on an insulin syringe) per administration. The calculator allows you to experiment with different BAC water volumes to find the most practical concentration for your application.
Can I use sterile water instead of bacteriostatic water?
Sterile water can be used to reconstitute a peptide, but it contains no preservative. A vial reconstituted with plain sterile water should be used within 24â48 hours to minimise the risk of bacterial contamination. For multi-use vials intended for research over days or weeks, bacteriostatic water is strongly preferred.
Need bacteriostatic water?
We stock both 3 mL and 10 mL vials of pharmaceutical-grade bacteriostatic water, shipped with cold-chain packaging.
Shop Bacteriostatic Water