Latest Posters

A Novel Fluorimetric Assay for the Detection of TACE (a-Secretase)
Vera Rakhmanova, Xudong Zhu, Fengying Li, Xiaofen Zhong, Anita Hong and Xiaohe Tong, AnaSpec, Inc.,
To facilitate high throughput screening of TACE inhibitors, AnaSpec synthesized a novel peptide substrate for TACE using QXL™ 520/5-FAM FRET pair. Using this FRET substrate, AnaSpec developed a new kit – the SensoLyte™ 520 TACE Activity Assay. This kit can be used to detect the activity of the enzyme and for screening of TACE inhibitors. It is highly sensitive and can detect subnanogram amounts of enzyme.

Involvement of enkephalins and delta-opioid receptors in the expression of levodopa-induced dyskinesia in a model of hemiparkinsonian rat
Fabrice Billet, Jean Costentin & Nathalie Dourmap, Laboratory of Experimental Neuropharmacology,
This study was performed to test the involvement of d-opioid receptors in dyskinesia induced by L-DOPA in hemiparkinsonian rats. Our results indicate that endogenous enkephakins modulate the expression of L-DOPA-induced dyskinesia. It also appears that d-opioid receptors located on corticostriatal terminals could be involved in the development of these dyskinesia. These results confer on d-opioid antagonists interesting properties in the improvement of Parkinson’s disease therapy.

Palladium-Catalyzed Three-Component Synthesis of Phenothiazines
Troels Dahl, Christian Wenzel Tornøe, Benny Bang-Andersen, Poul Nielsen and Morten Jørgensen
H. Lundbeck A/S

The formation of aromatic carbon-heteroatom bonds has traditionally been achieved by nucleophilic aromatic substitution or via the copper-mediated Ullman reaction. The palladium-catalyzed formation of aromatic C-N bonds extensively developed by Hartwig and Buchwald has provided a powerful alternative. While the aryl amination reaction is applicable even to aryl chlorides and activated phenols, the analogous C-O and C-S bond forming reactions have attracted less attention.

Decolorization of a Vancomycin Process Stream
Jon Fisher, Amie Gehris and John Maikner, Rohm and Haas Company,
Many process streams contain impurities generally given the name “color species”. These “color species” can foul resins in the upstream portion of purification processes, and must be removed in order to avoid quality problems with the pharmaceutical product.

Two-Dimensional Molecular Profiling of Multiple Myeloma
Zelena Jana, Konecna Hana, Zdrahal Zbynek, Penka Miroslav and Hajek Roman
Faculty Hospital Brno and Masaryk University

We have compared two different solubilization buffers, we also evaluated protein precipitation with ethanol and optimized 2-DE conditions for human myeloma proteins.

MS-Xelerator™: Advanced Algorithms for LC/MS Data Processing Applied to Biomarker Discovery, Differential Analysis and Quantitative Proteomics
M.M.A. Ruijken, MsMetrix,
LC-MS based proteomic experiments are used to compare complex biological samples across multiple conditions. Fast, powerful computational tools are needed to explore and detect differences in the areas of Expression Proteomics and Biomarker Discovery. In general, specialized steps are necessary to solve these difficult problems (binning, alignment & normalization, peak picking, relative quantitation, etc.). MS-Xelerator is a collection of software tools dedicated to all of the above tasks.